Guests at the Melbourne Museum can enjoy a variety of experiences. They can engage with touch screens, enter immersive environments, watch short films, join guided tours, or simply relax in one of the tranquil galleries. The museum features several distinct sections, such as the Children’s Gallery, known for its interactive, play-based exhibits, the Te Vainui O Pasifika Gallery, which represents Polynesian cultures and artworks; and the Phar Lap exhibit, showcasing Australia's legendary racehorse.

A standout feature of the museum is the Bunjilaka Aboriginal Cultural Centre, which offers visitors a deep understanding of Aboriginal social and cultural heritage. The remarkable living history project 'Birrarung,' highlights the work of artist Maree Clarke and immerses visitors in the ritual and ceremonial life of the Kulin Nation. Additionally, the Melbourne Gallery hosts a world-renowned collection that presents the city's rich history. Other notable areas include the Forest Gallery, the Science and Life Gallery, the Dynamic Earth exhibit, and the Evolution Gallery, each providing fascinating insights into the natural world that entertain and educate.